Cytometry

The technical platform CYTOMETRIE aims to study cell characteristics. The determination of these characteristics allows the phenotyping of cell populations as well as the evaluation of numerous cellular functions. The characteristics studied in the laboratory can be both descriptive (size, complexity, etc.) and functional, notably through natural fluorescence or that linked to the incorporation of probe molecules. For example, it is possible to study the relative size and complexity of cells nucleic acid content ; intracellular pigments (chlorophyll, phycoerythrin...) ; mitochondrial membrane potential ; lipid content ; cell viability ; signs of apoptosis manifestations ; intracellular oxidative activity ; phagocytosis ; multi-xenobiotic resistance ... The cells or models studied in the laboratory are varied: shellfish hemocytes (clams, oysters, clams, abalones, etc.), sperm and oyster oocytes, marine phytoplankton cells, toxic or not, marine bacteria, marine viruses, fish blood cells, etc. LEMAR has three main types of tools to evaluate these cellular characteristics, often complementary: flow cytometry, epifluorescence microscopy and flow imaging. Flow cytometry is a technique for analyzing cells suspended in a liquid flow. After excitation by a laser beam, the cells then diffuse or emit light signals. Quantifying these signals reveals their optical characteristics. Flow cytometry is a qualitative and quantitative technique that can process up to 10,000 events per second. Epifluorescence microscopy is a microscopic observation technique that takes advantage of the fluorescence of objects, allowing a specific structure, compound and/or activity to be visualized. Epifluorescence microscopy, combined with the possibility of optical cuts (pseudo-confocal technique), makes it possible to image objects in three dimensions. Stream imaging is a technique for acquiring images of objects measuring from a few microns to 2 mm present in a liquid stream. Image acquisition can be triggered by the presence of a fluorescent signal. An image analysis software coupled to this system allows the selection of objects of interest and the measurement of many descriptive parameters (diameter, perimeter, volume, circularity, concentrations...) [embed]https://www.youtube.com/watch?v=1DrKAJdKQAg&t=7s[/embed]

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