TY - JOUR TI - Fine-scale temporal dynamics of herpes virus and vibrios in seawater during a polymicrobial infection in the Pacific oyster Crassostrea gigas AU - Petton, Bruno AU - de Lorgeril, Julien AU - Mitta, Guillaume AU - Daigle, Gaetan AU - Pernet, Fabrice AU - Alunno-Bruscia, Marianne T2 - Diseases of Aquatic Organisms AB - The Pacific oyster Crassostrea gigas is currently being impacted by a polymicrobial disease that involves early viral infection by ostreid herpesvirus-1 (OsHV-1) followed by a secondary bacterial infection leading to death. A widely used method of inducing infection consists of placing specific pathogen-free oysters ('recipients') in cohabitation in the laboratory with diseased oysters that were naturally infected in the field ('donors'). With this method, we evaluated the temporal dynamics of pathogen release in seawater and the cohabitation time necessary for disease transmission and expression. We showed that OsHV-1 and Vibrio spp. in the seawater peaked concomitantly during the first 48 h and decreased thereafter. We found that 1.5 h of cohabitation with donors was enough time to transmit pathogens to recipients and to induce mortality later, reflecting the highly contagious nature of the disease. Finally, mortality of recipients was associated with increasing cohabitation time with donors until reaching a plateau at 20%. This reflects the cumulative effect of exposure to pathogens. The optimal cohabitation time was 5-6 d, the mortality of recipients occurring 1-2 d earlier. DA - 2019/// PY - 2019 DO - 10.3354/dao03384 DP - Web of Science VL - 135 IS - 2 SP - 97 EP - 106 J2 - Dis. Aquat. Org. LA - English SN - 0177-5103 UR - https://www.int-res.com/abstracts/dao/v135/n2/p97-106/ KW - ACL KW - Aquaculture KW - Bivalve KW - DECIPHER_ANR-14-CE19-0023 KW - Epidemiology KW - GIGASSAT_ANR-12-AGRO-0001 KW - Health KW - Ifremer KW - OPOPOP_ANR-13-ADAP-0007 KW - Pacific oyster mortality syndrome KW - Polymicrobial disease KW - REVENGE_ANR-16-CE32-0008 KW - disease-induced mortality KW - exposure KW - france KW - juvenile KW - microvariant KW - oshv-1 KW - oshv-1 mu-var KW - ostreid herpesvirus-1 KW - panorama KW - pathogen KW - poms KW - spat KW - survival ER -