TY - JOUR
TI - Molecular Characterization of Voltage-Gated Sodium Channels and Their Relations with Paralytic Shellfish Toxin Bioaccumulation in the Pacific Oyster Crassostrea gigas
AU - Boullot, Floriane
AU - Castrec, Justine
AU - Bidault, Adeline
AU - Dantas, Natanael
AU - Payton, Laura
AU - Perrigault, Mickael
AU - Tran, Damien
AU - Amzil, Zouher
AU - Boudry, Pierre
AU - Soudant, Philippe
AU - Hégaret, Hélène
AU - Fabioux, Caroline
T2 - Marine Drugs
AB - Paralytic shellfish toxins (PST) bind to voltage-gated sodium channels (Nav) and block conduction of action potential in excitable cells. This study aimed to (i) characterize Nav sequences in Crassostrea gigas and (ii) investigate a putative relation between Nav and PST-bioaccumulation in oysters. The phylogenetic analysis highlighted two types of Nav in C. gigas: a Nav1 (CgNav1) and a Nav2 (CgNav2) with sequence properties of sodium-selective and sodium/calcium-selective channels, respectively. Three alternative splice transcripts of CgNav1 named A, B and C, were characterized. The expression of CgNav1, analyzed by in situ hybridization, is specific to nervous cells and to structures corresponding to neuromuscular junctions. Real-time PCR analyses showed a strong expression of CgNav1A in the striated muscle while CgNav1B is mainly expressed in visceral ganglia. CgNav1C expression is ubiquitous. The PST binding site (domain II) of CgNav1 variants possess an amino acid Q that could potentially confer a partial saxitoxin (STX)-resistance to the channel. The CgNav1 genotype or alternative splicing would not be the key point determining PST bioaccumulation level in oysters.
DA - 2017/01//
PY - 2017
DO - 10.3390/md15010021
DP - Web of Science
VL - 15
IS - 1
SP - 21
J2 - Mar. Drugs
LA - English
SN - 1660-3397
UR - https://archimer.ifremer.fr/doc/00368/47877/
DB - https://www.mdpi.com/1660-3397/15/1/21
KW - Alexandrium minutum
KW - Crassostrea gigas
KW - ACCUTOX_ANR-13-CESA-0019
KW - ACL
KW - Alexandrium minutum
KW - CNRS
KW - Crassostrea gigas
KW - Ifremer
KW - LabexMER_ANR-10-LABX-0019
KW - Paralytic shellfish toxins
KW - SUD
KW - UBO
KW - adaptive evolution
KW - algal toxins
KW - alternative splicing
KW - amino-acid-sequence
KW - drosophila
KW - gene
KW - genomic organization
KW - german-cockroach
KW - ion channels
KW - na+ channel
KW - panorama
KW - paralytic shellfish toxins
KW - protein
KW - sodium channel
ER -