TY - JOUR TI - Molecular Characterization of Voltage-Gated Sodium Channels and Their Relations with Paralytic Shellfish Toxin Bioaccumulation in the Pacific Oyster Crassostrea gigas AU - Boullot, Floriane AU - Castrec, Justine AU - Bidault, Adeline AU - Dantas, Natanael AU - Payton, Laura AU - Perrigault, Mickael AU - Tran, Damien AU - Amzil, Zouher AU - Boudry, Pierre AU - Soudant, Philippe AU - Hégaret, Hélène AU - Fabioux, Caroline T2 - Marine Drugs AB - Paralytic shellfish toxins (PST) bind to voltage-gated sodium channels (Nav) and block conduction of action potential in excitable cells. This study aimed to (i) characterize Nav sequences in Crassostrea gigas and (ii) investigate a putative relation between Nav and PST-bioaccumulation in oysters. The phylogenetic analysis highlighted two types of Nav in C. gigas: a Nav1 (CgNav1) and a Nav2 (CgNav2) with sequence properties of sodium-selective and sodium/calcium-selective channels, respectively. Three alternative splice transcripts of CgNav1 named A, B and C, were characterized. The expression of CgNav1, analyzed by in situ hybridization, is specific to nervous cells and to structures corresponding to neuromuscular junctions. Real-time PCR analyses showed a strong expression of CgNav1A in the striated muscle while CgNav1B is mainly expressed in visceral ganglia. CgNav1C expression is ubiquitous. The PST binding site (domain II) of CgNav1 variants possess an amino acid Q that could potentially confer a partial saxitoxin (STX)-resistance to the channel. The CgNav1 genotype or alternative splicing would not be the key point determining PST bioaccumulation level in oysters. DA - 2017/01// PY - 2017 DO - 10.3390/md15010021 DP - Web of Science VL - 15 IS - 1 SP - 21 J2 - Mar. Drugs LA - English SN - 1660-3397 UR - https://archimer.ifremer.fr/doc/00368/47877/ DB - https://www.mdpi.com/1660-3397/15/1/21 KW - Alexandrium minutum KW - Crassostrea gigas KW - ACCUTOX_ANR-13-CESA-0019 KW - ACL KW - Alexandrium minutum KW - CNRS KW - Crassostrea gigas KW - Ifremer KW - LabexMER_ANR-10-LABX-0019 KW - Paralytic shellfish toxins KW - SUD KW - UBO KW - adaptive evolution KW - algal toxins KW - alternative splicing KW - amino-acid-sequence KW - drosophila KW - gene KW - genomic organization KW - german-cockroach KW - ion channels KW - na+ channel KW - panorama KW - paralytic shellfish toxins KW - protein KW - sodium channel ER -