TY - JOUR TI - High temperature induces transcriptomic changes in Crassostrea gigas that hinder progress of ostreid herpesvirus (OsHV-1) and promote survival AU - Delisle, Lizenn AU - Pauletto, Marianna AU - Vidal-Dupiol, Jeremie AU - Petton, Bruno AU - Bargelloni, Luca AU - Montagnani, Caroline AU - Pernet, Fabrice AU - Corporeau, Charlotte AU - Fleury, Elodie T2 - Journal of Experimental Biology AB - Of all environmental factors, seawater temperature plays a decisive role in triggering marine diseases. Like fever in vertebrates, high seawater temperature could modulate the host response to pathogens in ectothermic animals. In France, massive mortality of Pacific oysters, Crassostrea gigas, caused by the ostreid herpesvirus 1 (OsHV-1) is markedly reduced when temperatures exceed 24 degrees C in the field. In the present study we assess how high temperature influences the host response to the pathogen by comparing transcriptomes (RNA sequencing) during the course of experimental infection at 21 degrees C (reference) and 29 degrees C. We show that high temperature induced host physiological processes that are unfavorable to the viral infection. Temperature influenced the expression of transcripts related to the immune process and increased the transcription of genes related to the apoptotic process, synaptic signaling and protein processes at 29 degrees C. Concomitantly, the expression of genes associated with catabolism, metabolite transport, macromolecule synthesis and cell growth remained low from the first stage of infection at 29 degrees C. Moreover, viral entry into the host might have been limited at 29 degrees C by changes in extracellular matrix composition and protein abundance. Overall, these results provide new insights into how environmental factors modulate host-pathogen interactions. DA - 2020/10// PY - 2020 DO - 10.1242/jeb.226233 DP - Web of Science VL - 223 IS - 20 SP - jeb226233 J2 - J. Exp. Biol. LA - English SN - 0022-0949 KW - ACL KW - Anti-viral molecular pathway KW - Host-pathogen interaction KW - Ifremer KW - Marine disease KW - OsHV-1 KW - Resistance KW - Temperature KW - VIVALDI_H2020-SFS-2015-2 (678589) KW - adaptation KW - differential expression KW - fatty-acid-composition KW - hemocyte parameters KW - infected pacific oyster KW - management KW - metabolism KW - mortalities KW - panorama KW - purification KW - water temperature ER -