Post-doctorate (M/F) in Molecular Biology and Bioinformatics
Within the framework of ANR PARASED, the post-doctorate is funded by the Brittany region as part of its SAD scheme.
The work of the postdoctoral fellow, funded by the Brittany region, will consist in conducting molecular biology experiments and analysing high-speed transcriptomics and genomics data to understand the plasticity of the virulence mechanisms of invasive eukaryotic marine parasites of bivalves belonging to the genus Perkinsus (Perkinsozoa, Alveolata).
Using a new targeted transcriptome methodology developed at LEMAR, this project proposes to evaluate the infection plasticity of P. olseni during in situ infection of infected tissues of several hosts in a model site, the Rade de Brest. In this context, this post-doctoral work will focus on 2 questions: 1) is the targeted transcriptome representative of the global expression of parasite genes expressed in the infected tissues and 2) how can P. olseni infect two hosts of the same Ruditapes species (one invasive and one native).
Objective 1: Validation of the methodology. In a first step, the post-doctoral fellow will validate the methodology on trophozoite stages in culture by performing a global and targeted transcriptome in illumina sequencing. All generated sequences will be assembled, annotated and incorporated into a metabolic reconstruction using KEGG comparisons to identify probable gains/losses and significant regulation of parasitic metabolic pathways. A comparison of these data sets will reveal the representativeness of the target transcriptome relative to the overall transcriptome. These data are already available for analysis. A bioinformatics analysis pipeline will then be created for the analysis of the objective 2 data (collaboration A. Monier U. Exeter).
Objective 2: Analysis of targeted transcriptomes in infected tissues of two Ruditapes host species (one invasive and one native). The post-doctoral fellow will be expected to perform RNA extractions and purifications, sequence the targeted transcriptomes and perform bioinformatics analysis using the pipeline created in objective 1.
The candidate must have defended his/her thesis for less than 2 years and must have spent at least 18 months abroad. The candidate should be proficient in molecular biology techniques (e.g. DNA and RNA extraction) and be autonomous in bioinformatics analysis in genomics and transcriptomics (e.g. a good knowledge of Unix/Linux, R). A good level of English is also required.
18 mois (Contrat à durée déterminée)
Localisation du post-doctorant au LEMAR, UMR 6539 à Brest au sein de l'équipe PAnorama.; 38h30/semaine; quelques déplacements dans l'équipe du A. Monier (Université d'Exeter, Angleterre) sont prévus pour compléter les analyses bio-informatiques.
From 2643.22€ to 4207.80€ gross salary, according to experience